Helios

• Proteomic CyTOF system Helios enables detection and quantification of more than 40 protein markers in individual cells
• Unique metal Maxpar antibodies provide simultaneous profiling of intracellular, extracellular and phosphorylated proteins in a single cell without the risk of overlapping spectra between detection channels
• Mass cytometry allows you to simultaneously monitor the phenotype and function of individual cells in your experimental system
• Instrument ionizes and releases metal tags from individual cells, separates them by weight and calculates how many cells have been bound to each cell
  
  
  

Fluidigm Maxpar reagents

• Individual cells in the cell suspension can be labeled with more than 40 metal conjugated isotopes from the Fluidim catalogue (more than 600 antibody) or an antibody of your choice either using a metal labeling kit or you can use the custom conjugation service Fluidigm
• Ready-to-use validated antibody-labeled panels against various cellular targets are available

Cell profiling in suspension or in resuspended tissue

• Cell surface
• Cytoplasm
• Nucleus
• Other organels
• DNA

Barcoding

• Ability to bind up to 20 unique metal tags to individual cell suspensions
• Multiplexing of rare low volume samples

Analysis can be performed on available cytometric sotwares and:

• Fluidigm CyTOF Software v6.7
• Data analysis software on cloud based Cytobank uses mass cytometry tools and lets you share data with collaborators
• Debarcoder v1.04