C1

• Automatic sample preparation system for the separation and isolation of individual cells with the following reverse transcript and nucleic acid amplification
• Prepared single-stranded templates may be used for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression
• In comparison with 384 PCR format, C1 generates 24x more data
• By concentrating and automating individual steps into one device it is possible to reduce the variability caused by the classical use of different platforms for each step
• As with most Fluidigm systems, the operation of this device is based on the principle of microfluidic technology
• Instrument isolates individual cells into individual reaction chambers (IFCs)
• Optically clear IFCs allow to control individual fluorescently labeled cells under the microscope, it is possible to determine their lifetime or to follow the labeled surface markers
• Cells are then automatically lysed and processed and the template can be used for qPCR or sequencing analysis
• Entire process takes less than 4 hours

Advantages of C1 instrument:

• ACCURACY: the ability to detect differences in heterogeneous populations with individual cell accuracy
• SIMPLICITY: simple isolation process of individual cells with clearly defined experimental workflow
• SPEED: minimized operator time due to automation and very reduced manual pipetting time
• FLEXIBILITY: pre-prepared applications for RNA and DNA analysis or build your own protocol on the C1 Open App (https://www.fluidigm.com/c1openapp)

For more information see here.