Amplicon target enrichment panels

Target enrichment is a technology that allows selective sequencing of selected regions of the genome, often regions where clinically relevant nucleotide polymorphisms (mutations) are found. Compared to whole genome sequencing, it is much more time and cost efficient.
The technology of amplicon target enrichment is based on the use of forward and reverse primers that delineate the regions of interest and allow these regions to be amplified by PCR. The resulting amplicons then enter the subsequent NGS process.

Target enrichment using amplicons serves similar purposes to target enrichment based on hybridisation capture, but is more suitable for detecting clinically relevant gene variants at loci that are close together. Compared to hybridization capture, it is much cheaper and less time consuming. For the automated preparation of amplicon libraries, we present in this section a solution from Fluidigm.


The Juno instrument uses microfluidic technology, specifically special IFC (integrated fluidic circuit) plates that allow the preparation of amplicon libraries within a very fast and simple protocol.


The following Advanta NGS kits are available for this platform:

Advanta RNA-Seq NGS Library Prep Kit
RNA sequencing (RNA-seq) is the gold standard for tracking gene expression and is a fundamental approach for molecular biology laboratories. The Advanta RNA-Seq NGS Library Prep Kit, together with the Juno™ system, is designed to enable a significant improvement in RNA sequencing workflow, providing an integrated solution for automated and cost-effective NGS library preparation.

Advanta Solid Tumor NGS Library Prep Assay
Analyze multiple variants with a comprehensive panel of 53 important genes that support the diagnosis of multiple cancers and solid tumors.

Advanta RNA Fusions NGS Library Prep Assay
Kit covers more than 380 gene fusion pairs and over 1,000 known breakpoints in solid tumors and hematological cancers. It provides important data for cancer research and allows simultaneous analysis of RNA and DNA variants from up to 6 unique panels of assays in a single run.

Advanta CFTR NGS Library Prep Assay
Kit provides a highly efficient and scalable NGS library preparation workflow on the automated Juno system. The assay is designed to detect target CFTR gene variants in each of the 27 exons and selected intron regions. It easily produces high-quality NGS-ready libraries with an index for significantly shorter run times.